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Live-cell tracking of PAR formation in response to H 2 O 2 . Time course images of the live-cell assay shown in <xref ref-type=Figure 2C . HeLa cells subjected to PAR-T ddGFP expression were treated with 20 µM PJ34 for 2 hr prior to 15 min of treatment with 1 mM H 2 O 2 and live-cell imaging. The 15-min time course of H 2 O 2 treatment was condensed to the time frame shown in the video (1-min sampling interval, 15 frames total). The scale bar is 5 µm. " width="250" height="auto" />
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Live-cell tracking of PAR formation in response to H 2 O 2 . Time course images of the live-cell assay shown in <xref ref-type=Figure 2C . HeLa cells subjected to PAR-T ddGFP expression were treated with 20 µM PJ34 for 2 hr prior to 15 min of treatment with 1 mM H 2 O 2 and live-cell imaging. The 15-min time course of H 2 O 2 treatment was condensed to the time frame shown in the video (1-min sampling interval, 15 frames total). The scale bar is 5 µm. " width="100%" height="100%">

Journal: eLife

Article Title: Development and characterization of new tools for detecting poly(ADP-ribose) in vitro and in vivo

doi: 10.7554/eLife.72464

Figure Lengend Snippet: Live-cell tracking of PAR formation in response to H 2 O 2 . Time course images of the live-cell assay shown in Figure 2C . HeLa cells subjected to PAR-T ddGFP expression were treated with 20 µM PJ34 for 2 hr prior to 15 min of treatment with 1 mM H 2 O 2 and live-cell imaging. The 15-min time course of H 2 O 2 treatment was condensed to the time frame shown in the video (1-min sampling interval, 15 frames total). The scale bar is 5 µm.

Article Snippet: The plasmids for Dox-inducible expression of the ddGFP PAR-T constructs were generated using a cDNA for ddGFP-A (Addgene, 40286) or ddGFP-B (Addgene, 40287). cDNAs for the PAR binding domains were amplified from previously published pET19b constructs ( ).

Techniques: